Analysis of Lys aminoacyl synthetase.

Analysis performed on 2024-07-10 11:39:22

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 44328 601 43727 21938
Archaea 3239 87 3152 311
Vertebrata 978 277 701 416
Mammals 354 51 303 130

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
597 82 651

Domains

Domains
Current Study 1, 61
Guo et al (2010) 20, 40

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 537 60 597 8.950000
Number of Variants 647 86 733 7.523256
Number of Pathogenic Variants 79 3 82 26.333333
Number of Benign/Unknown Variants 568 83 651 6.843373
Fisher exact test 0.016297

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 576 21 597 27.428571
Number of Variants 716 17 733 42.117647
Number of Pathogenic Variants 81 1 82 81.000000
Number of Benign/Unknown Variants 635 16 651 39.687500
Fisher exact test 0.709329

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 537 60 0.92 (0.01) 0.71 (0.03) 9.809e-01 7.380e-01 26382.50 2.38e-16
Guo et al (2010) 576 21 0.90 (0.01) 0.90 (0.03) 9.771e-01 9.577e-01 6946.50 1.23e-01

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains